While delivering his remarks, Macron said "all lives have equal worth." Fighting terrorism "can never be carried out without rules," he added.
The gathering in Paris brought together officials from Western and Arab nations, the United Nations and nongovernmental organizations, with the aim of providing urgent aid to civilians in the Gaza Strip that is being pounded by Israel in its war against Hamas.
Israeli authorities weren't invited but have been informed of the talks, Macron's office said.
Macron reiterated calls for a humanitarian pause in Israel's military operation in Gaza.
Despite the calls, Macron defended Israel's right to defend itself from the militant group.
Hamas "shouldered the responsibility for exposing Palestinians to terrible consequences," he said.
The French leader also stressed that civilians must be protected.
"It's absolutely essential. It is non-negotiable," he said. "All lives have equal worth and there are no double standards for those of us with universal and humanist values."
"Fighting terrorism can never be carried out without rules. Israel knows that. The trap of terrorism is for all of us the same: giving in to violence and renouncing our values," Macron added.
More than 1.5 million people — or about 70% of Gaza's population — have fled their homes, and an estimated $1.2 billion is needed to respond to the crisis in Palestinian areas.
Longer term, Macron said diplomatic work must resume on bringing peace to the Middle East, with a two-state solution.
"We must learn from our errors and no longer accept that peace in the Middle East always be pushed back to later," he said.
Paris says it is considering evacuating injured people to hospital ships in the Mediterranean off the Gaza coast.
Thursday's discussions also included financial support to help Gazans.
Macron said his nation will provide an additional $85 million in humanitarian aid for Gaza, bringing France's funding to a total of $107 million this year.
Denmark said it will increase its humanitarian aid to the civilian population in Gaza by $10.7 million, to be channeled via U.N. agencies and the International Red Cross.
European Commission President Ursula von der Leyen who was among the attendees said the bloc has "quadrupled the humanitarian support for Gaza and the West Bank, but it's mostly for Gaza, to $107 million."
The 27-nation bloc is the world's top aid supplier to the Palestinians.
On Tuesday, Germany said it will provide $21 million in new funding, in addition to releasing $76 million already earmarked for the U.N. agency for Palestinian refugees following a review it launched after the Hamas attack.
The Gaza aid conference had an attendance from over 50 nations, including several European countries, the United States and regional powers such as Jordan, Egypt and the Gulf countries.
Speaking at the conference, Palestinian Prime Minister Mohammad Shtayyeh, who urged the international community to "put an end to the war" asked "how many Palestinians have to be killed for the war to end ?"
Shtayyeh said "what Israel is doing is not a war against Hamas, it's a war against the whole Palestinian people."
"We must take care of the wounded, provide electricity, water, medicines," he added.
Egypt's Foreign Minister Sameh Shoukry stressed that Israel had only allowed limited quantities of humanitarian aid through the Rafah crossing and urged "the entire international community, and donor countries in particular, to continue supporting the Palestinian people in Gaza."
"The aid that has already entered Gaza is not enough to meet the needs of the entire population, and the voluntary and deliberate complications imposed by Israel on the delivery of aid only lead to a further deterioration of the situation," he said.
Cyprus President Nikos Christodoulides outlined his plan for a humanitarian sea corridor to Gaza "to provide continued rapid, safe and unhindered flow of humanitarian aid in a pragmatic and effective manner."
He said the plan is being discussed "with all parties concerned, including Israel" and provides options for the short, medium and longer term.
Ships would deliver the aid from Cyprus' main port of Limassol.
Ahead of the conference, Amnesty International called on states "to push for an immediate cease-fire by all parties — as this is a vital precondition to ensure the people of Gaza receive any kind of sustained, effective, and impactful humanitarian aid."
On Wednesday evening, human rights activists from several groups including Doctors Without Borders, Action Against Hunger and Doctors of the World gathered near the Eiffel Tower to call for an immediate cease-fire in Gaza.
"If the parties involved in this conference only discuss technical details such as the number of trucks to be driven through, it will amount to a cosmetic discussion that will delay the real issue, the cease-fire," Michel Lacharité, the head of emergency operations at Doctors without Borders France, said in a statement.