The Masai indigenous community has lived in the Ngorongoro Reserve, a UNESCO World Heritage site in northern Tanzania, for over a century.
But they now face being moved out, as the authorities contend that their growing population is a threat to wildlife habitat.
Officials insist the relocation is voluntary.
Arusha regional commissioner John Mongella said around 296 families had registered for the move to Handeni, a district 600 kilometers (370 miles) south of Ngorongoro.
"There is no eviction here, all people who are leaving (are) voluntarily registered and the government is facilitating them," he said in a video statement.
The government has earmarked 162,000 hectares (400,000 acres) of land for relocated Maasai households, he said.
But the community remains sharply divided over the issue, with many reluctant to leave the only home they have ever known.
"This eviction has never been voluntary for Ngorongoro people," Ngorongoro-based human rights lawyer and activist Joseph Oleshangay told AFP.
A handful of families packing up for the move expressed cautious optimism over the scheme.
"I have seen water, schools and even electricity in the new residence, why shouldn't I go there?" said Edward Sapuru, a herder.
The relocation has sparked concern, with a team of UN-appointed independent rights experts warning that "it could jeopardize the Maasai's physical and cultural survival."
"This will cause irreparable harm, and could amount to dispossession, forced eviction and arbitrary displacement prohibited under international law," they said in a statement on Wednesday.
Tanzania has historically allowed indigenous communities such as the Maasai to live within some national parks.
But since 1959, the number of humans living in Ngorongoro has shot up from 8,000 to more than 100,000.
The Maasai say the authorities are attempting to force them off their land in order to organise safaris and private hunting expeditions for tourists.
The government has rejected these accusations, but the issue has led to clashes between the pastoralists and police.